In Vitro Adventitious Rooting of Carrizo Citrange Microshoots
Identifieur interne : 001C73 ( Main/Exploration ); précédent : 001C72; suivant : 001C74In Vitro Adventitious Rooting of Carrizo Citrange Microshoots
Auteurs : Almudena Montoliu [Espagne] ; Aurelio Gomez-Cadenas [Espagne] ; Rosa M. Perez-Clemente [Espagne]Source :
- HortScience [ 0018-5345 ] ; 2010.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Horticulture, Biotechnologie.
English descriptors
- KwdEn :
Abstract
The objective of this work was to develop an efficient in vitro rooting protocol for one of the most commercially used citrus rootstocks in Spain, Carrizo citrange (Citrus sinensis L. Osbeck x Poncirus trifoliata L. Raf.). Single-node cuttings taken from greenhouse-grown plants were cultured in petri dishes containing basal Murashige and Skoog medium. Shoots from nodal stem segments were excised and cultured in a multiplication medium (basal medium supplemented with 1.8 μM 6-benzylaminopurine) to promote the development of axillary buds. Individual shoots (15 mm long) were treated with different hormones at several concentrations for root induction evaluations. The addition of activated charcoal (AC) to the culture medium was also explored. The addition of auxins to the culture medium enhanced rooting percentage. Optimal results were obtained when 1-naphthalene acetic acid (10.8 μM) and gibberellic acid (0.3 μM) were added to the culture medium. The addition of AC to the rooting medium resulted in negative effects on the percentage of rooted shoots but had a positive effect on number of roots per rooted shoot. Chemical names used: activated charcoal (AC); 6-benzylaminopurine (BA); 1-naphthalene acetic acid (NAA); gibberellic acid (GA3); indole-3-butyric acid (IBA).
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Perez-Clemente</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Laboratorio de Ecofisiología y Biotecnología, Departamento de Ciencias Agrarias y del Medio Natural, Universitat Jaume I</s1><s2>12071 Castellón de la Plana</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">10-0346043</idno><date when="2010">2010</date><idno type="stanalyst">PASCAL 10-0346043 INIST</idno><idno type="RBID">Pascal:10-0346043</idno><idno type="wicri:Area/PascalFrancis/Corpus">000230</idno><idno type="wicri:Area/PascalFrancis/Curation">000770</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000229</idno><idno type="wicri:doubleKey">0018-5345:2010:Montoliu A:in:vitro:adventitious</idno><idno type="wicri:Area/Main/Merge">001D13</idno><idno type="wicri:Area/Main/Curation">001C73</idno><idno type="wicri:Area/Main/Exploration">001C73</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">In Vitro Adventitious Rooting of Carrizo Citrange Microshoots</title><author><name sortKey="Montoliu, Almudena" sort="Montoliu, Almudena" uniqKey="Montoliu A" first="Almudena" last="Montoliu">Almudena Montoliu</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Laboratorio de Ecofisiología y Biotecnología, Departamento de Ciencias Agrarias y del Medio Natural, Universitat Jaume I</s1><s2>12071 Castellón de la Plana</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Gomez Cadenas, Aurelio" sort="Gomez Cadenas, Aurelio" uniqKey="Gomez Cadenas A" first="Aurelio" last="Gomez-Cadenas">Aurelio Gomez-Cadenas</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Laboratorio de Ecofisiología y Biotecnología, Departamento de Ciencias Agrarias y del Medio Natural, Universitat Jaume I</s1><s2>12071 Castellón de la Plana</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Perez Clemente, Rosa M" sort="Perez Clemente, Rosa M" uniqKey="Perez Clemente R" first="Rosa M." last="Perez-Clemente">Rosa M. Perez-Clemente</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Laboratorio de Ecofisiología y Biotecnología, Departamento de Ciencias Agrarias y del Medio Natural, Universitat Jaume I</s1><s2>12071 Castellón de la Plana</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author></analytic><series><title level="j" type="main">HortScience</title><title level="j" type="abbreviated">HortScience</title><idno type="ISSN">0018-5345</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">HortScience</title><title level="j" type="abbreviated">HortScience</title><idno type="ISSN">0018-5345</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Activated carbon</term><term>Adventitious root</term><term>Auxin</term><term>Biotechnology</term><term>Citrange</term><term>Citroncirus webberi</term><term>Citrus</term><term>Horticulture</term><term>In vitro</term><term>Micropropagation</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>In vitro</term><term>Racine adventive</term><term>Multiplication végétative in vitro</term><term>Charbon actif</term><term>Horticulture</term><term>Biotechnologie</term><term>Citroncirus webberi</term><term>Citrus</term><term>Citrange</term><term>Auxine</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Horticulture</term><term>Biotechnologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objective of this work was to develop an efficient in vitro rooting protocol for one of the most commercially used citrus rootstocks in Spain, Carrizo citrange (Citrus sinensis L. Osbeck x Poncirus trifoliata L. Raf.). Single-node cuttings taken from greenhouse-grown plants were cultured in petri dishes containing basal Murashige and Skoog medium. Shoots from nodal stem segments were excised and cultured in a multiplication medium (basal medium supplemented with 1.8 μM 6-benzylaminopurine) to promote the development of axillary buds. Individual shoots (15 mm long) were treated with different hormones at several concentrations for root induction evaluations. The addition of activated charcoal (AC) to the culture medium was also explored. The addition of auxins to the culture medium enhanced rooting percentage. Optimal results were obtained when 1-naphthalene acetic acid (10.8 μM) and gibberellic acid (0.3 μM) were added to the culture medium. The addition of AC to the rooting medium resulted in negative effects on the percentage of rooted shoots but had a positive effect on number of roots per rooted shoot. Chemical names used: activated charcoal (AC); 6-benzylaminopurine (BA); 1-naphthalene acetic acid (NAA); gibberellic acid (GA<sub>3</sub>); indole-3-butyric acid (IBA).</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Communauté valencienne</li></region></list><tree><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Montoliu, Almudena" sort="Montoliu, Almudena" uniqKey="Montoliu A" first="Almudena" last="Montoliu">Almudena Montoliu</name></region><name sortKey="Gomez Cadenas, Aurelio" sort="Gomez Cadenas, Aurelio" uniqKey="Gomez Cadenas A" first="Aurelio" last="Gomez-Cadenas">Aurelio Gomez-Cadenas</name><name sortKey="Perez Clemente, Rosa M" sort="Perez Clemente, Rosa M" uniqKey="Perez Clemente R" first="Rosa M." last="Perez-Clemente">Rosa M. Perez-Clemente</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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